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| Measuring and Analysis of Complete Genome Sequencing of Shallot yellow stripe virus Isolate from Tillering Onion in Jilin，China |
| MA Jun-feng，ZHANG Chen，ZHANG Chun-yu，LI Xiao-yu，WANG Yong-zhi* |
| Institute of Plant Protection，Jilin Academy of Agricultural Sciences，Gongzhuling 136100，Jilin，China |
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Abstract The complete genome of Shallot yellow stripe virus（SYSV） Jilin isolate SYSV-JL（MN607702）
was cloned from SYSV infected tillering onion by RT-PCR．The complete sequence of SYSV-JL consisted of
10 427 nucleotides，and shared high sequence identity with 3 SYSV isolates that had submitted to GenBank
in many gene segments，but the sequence identity of nucleotide and amino acid in P1 gene was lower，
only sharing 69.5%-84.2% and 60.9%-76.9%，respectively．Further variation analysis showed that P1 gene
contained 369 variation sites，showing obvious high variation characteristics．Phylogenetic analysis indicated
that different SYSV isolates had distinct regional differences and the phylogenetic relationship between SYSV-JL
isolate and multiple Chinese isolates was closer．
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Received: 22 October 2019
Published: 08 April 2020
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