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| Screening of Resistant Germplasm of Tomato spotted wilt virus and Optimization of Artificial Identification Method |
| SHI Yan，MO Ning，QI Shi-ming，LIANG Yan* |
| College of Horticulture，Northwest A&F University，Yangling 712100，Shaanxi，China |
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Abstract In this study，154 tomato materials，including 76 common tomatoes and 78 cherry tomatoes
were investigated twice for the disease indexes of Tomato spotted wilt virus（TSWV）in fields for 2 years，
and 14 of them were screened with stable resistance to TSWV．Molecular marker identification was carried out
by Sw-5 co-dominant SCAR marker on materials with disease resistance in fields．Three materials carrying
Sw-5 gene against TSWV were discovered．In order to shorten the artificial inoculation identification period
for TSWV，this study took disease resistant material H8 containing Sw-5 and susceptible material M82 as
study object；set up 4 inoculation periods for 4，6，8 and 10 true leaf blades；and conducted disease index
investigation and resistance grading 14，21，28 days after inoculation．The results showed that inoculation
during 6 true leaf stage，and conducting disease investigation 28 days after inoculation could effectively
identify plant resistance for TSWV．Matching with the effect of inoculation in 8，10 true leaf blades stage，the
efficiency of artificial inoculation for disease resistance identification was significantly promoted．
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Received: 30 September 2019
Published: 08 November 2019
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