In order to clarify the effect of mapk two-stranded RNA expression carrier transgenic cucumber
on soil nematode diversity，this paper adopted 18S rDNA gene cloning library method and studied mapk twostranded
RNA expression carrier cucumber and diversity of soil nematode in rhizosphere of non-transgenic
cucumber. The results showed that the soil nematodes in rhizosphere of transgenic cucumber and non-transgenic
cucumber were mainly bacterial-feeding nematode including Rhabditis sp.，Cephalobus sp. and Acrobeloides
sp. There was no significant difference in diversity index and ecological index，indicating that there was no
significant influence of mapk double-stranded RNA expression carrier cucumber on soil nematode diversity. It
has higher biosecurity.