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| Preliminary Studies on The Function of BrZAT12，a Protein Interacted with BrVIN3.1，during Flowering Time in Chinese Cabbage |
| XIN Xiao-yun 1，WANG Bin 1，2，ZHAO Xiu-yun 1，ZHANG De-shuang 1，WANG Wei-hong 1，YU Yang-jun 1，SU Tong-bing 1，YU Shuan-cang 1，ZHANG Feng-lan 1* |
1 Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China；2College
of Plant Science and Technology，Beijing College of Agriculture，Beijing 102206，China |
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Abstract During flowering time process，BrVIN3.1 regulates by mediating the epigenetic states of
BrFLC1，regulated its expression then controlled the flowering time of Chinese cabbage．Taking BrVIN3.1
（Bra020445）as bait，the paper carried out screen selection on yeast cDNA library and obtained BrVIN3.1’s
interaction protein and BrZAT12（Bra006691）．Yeast-two-hybrid verification was conducted．The results
showed that they did interact in vivo．Chinese cabbage materials at different flowering times were treated by
refrigeration．Expression pattern was analyzed．The results showed that BrZAT12 expression patterns were
different in Chinese cabbage materials at different bolting times．The expression quantities of easy bolting
materials were higher than those of tolerant bolting materials，which had lesser amplitude of variation．In
summary，the paper speculated that BrZAT12 may function in flowering pathway by regulating the expression
of BrVIN3.1 in Chinese cabbage．
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Received: 21 February 2019
Published: 27 September 2019
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