Bitter gourd Fusarium wilt is a soil-borne disease caused by Fusarium oxysporum f. sp.momordicae．At present，better pesticides or biocontrol agents are in urgent need in market．In order to control bitter gourd Fusarium wilt with better effect，this paper conducted pot cultivation experiment，screened the optimal combination of Pseudomonas chlororaphis G5+hymexazol．The control effect of the optimal combination screened by pot experiment was verified in the pathogenic area of bitter gourd Fusarium wilt．The results showed that the combination B+0.75Hym（1×10⁹ cfu · mL^-1 G5 suspension+187.5 mg · L^-1 hymexazol）had 81.60% disease control effect on potted bitter gourd Fusarium wilt，which was higher than that by the other treatments．At the same time，the combination had the most obvious growth promoting effect on bitter gourd seedlings．After inoculation with Fusarium oxysporum，the activities of PAL，PPO and POD defense enzymes in bitter gourd leaves treated with combination B+0.75Hym were higher．The control effect in field experiment of combination B+0.75Hym on bitter gourd Fusarium wilt was 52.59% and 32.13%，respectively 30 days and 60 days after transplantation．And the yield of bitter gourd was also higher than that by the other treatments．These results showed that the optimum combination B+0.75Hym screened from pot experiment could not only reduce the application amount of pesticides，but also effectively control bitter gourd Fusarium wilt and increase bitter gourd yield．It has certain practical value in production．