Taking‘Kexin No.1’as experimental material，this paper studyied the effects of adding different
biochar quantities（10，20，40 t·hm^-2）on diversity of potato root rhizosphere soil and yield of potato by ITS
high-throughput sequencing technology．The results showed that all biochar treatments reduced the soil fungal
diversity，and the fungal abundance of T3 treatment（ 20 t·hm^-2） was the lowest．The potato rhizosphere soil
fungi mainly included 5 phyla，25 classes，62 orders，120 families，201 genera and 345 species．The dominant
fungi community at the phylum classification level was Ascomycota．Its relative abundance was 86.86%-95.86%．
Adding biochar treatment could increase the abundance of Ascomycota and reduce abundance of Basidiomycete
and Zygomycota．At the level of family taxonomy，adding biochar treatments could increase the abundance
of Sordariaceae Incertae sedis 23，Microascaceae，Chaetomiaceae，Nectriaceae and reduce the abundance
of Ascomycota，Sordariomycetes，Sordariales Incertae sedis．The potato yield，tuber number per plant and
commercial potato rate were significantly increased by adding biochar treatments．Among them T3 had significant
effect．By comprehensive analysis on yield index and fungi diversity，T3 treatment was most conducive to increase
potato yield and improve soil fertility．