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| Studies on Method for Improving Embryoid Induction Rate of Spring Watermelon Unfertilized Ovary |
| MIN Zi-yang1，2，ZOU Tian1，RUAN Wan-hui2，SUN Xiao-wu1* |
（1College of Horticulture and Landscape，Hunan Agricultural University，Changsha 410128，Hunan，China；2Hunan
Research Institute of Vegetables，Changsha 410125，Hunan，China） |
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Abstract In order to improve embryoid induction rate of watermelon unfertilized ovary and accelerate its
haploid breeding process，this experiment took unfertilized ovary of watermelon‘ Zaojia8428’ as material and
studied on the factors affecting induction rate of embryoid grown in spring．The results showed that keeping the
fruit stalk as long as possible when the ovary was taken，then steeping it in 0.02 mg·L-1 TDZ for 10 min and
putting it at 15 ℃ as pretreatment for 2 days could improve the induction rate of embryoid from 3.5% to 15.9%．
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Received: 09 November 2017
Published: 28 February 2018
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