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| Establishment of Resistance Evaluation System for Bacterial Spot of Processing Tomato and Screening of Biological Agents |
| GUO Wei-tao1，2，ZHOU Jun-guo1*，WU Chang-liu2，CHAI A-li2，LI Bao-ju2* |
| （1Henan Institute of Science and Technology，Xinxiang 45300，Henan，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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Abstract In order to establish a rapid and effective technology to identify resistance to bacterial spot
disease of processing tomato，this paper compared 5 innoculation methods，including spraying，smear，axillary
acupuncture，acupuncture on stem，and root irrigation on tomato bacterial spot. The results showed that spraying
was the best inoculation method. It was simple and effective，suitable for resistance identification on germplasm
resources. A total of 209 Solanum pimpinellifolium germplasm resources were evaluated by spray inoculation
method. Among them，22 materials were mid-resistant to diseases，57 were susceptible，130 were high
susceptible，and no resistant or immune materials were found. By indoor pot experiment，23 kinds of biocides were
evaluated for their prevention and control effect on tomato bacterial spot. There were 2 kinds of safe and efficient
biological agents，namely spore IVF 018 and spore IVF 021，respectively. The preventive and curative effects on
bacterial spot disease were 100.00%，82.50% and 71.25%，61.67%，respectively. And the prevention effect was
obviously higher than curative effects，indicating prevention and curative in fields should take prevention first.
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Received: 07 February 2018
Published: 20 March 2018
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