The 284 cucumber samples affected by virus in the fields of Shunyi and Changping Districts
of Beijing，Shouguang of Shandong Province，and Shenzhen of Guangdong Province，were identified on their
species of virus diseases and detected them with virus antibodies against 5 cucumber viruses，including Cucumber
mosaic virus，Watermelon mosaic virus，Zucchini yellow mosaic virus，Cucumber green mottled mosaic virus
and Papaya ring spot virus by ELISA method．The results showed that CMV，WMV，ZYMV were detected on the infected leaves of 126 and 124 samples collected in Shunyi and Changping Districts of Beijing．Among
them the detection rate of CMV was the highest，all was 100.00% in Shunyi and Changping．The detection rate
of ZYMV were 80.95% and 66.94%，respectively．The detection rate of WMV was the lowest - 51.59% and
53.22%，respectively．Only CMV was detected on the infected fruit．CMV，WMV，ZYMV，PRSV 4 viruses
were detected on the infected leaves of 28 samples collected in Shouguang of Shandong Province．The detection
rates of these 4 viruses were 100.00%，35.71%，35.71% and 17.86%，respectively．CMV，WMV，PRSV
were detected on the infected leaves of 6 infected cucumber samples collected in Shenzhen．The detection rates
were 100.00%，50.00% and 50.00%，respectively．Investigation discovered that the phenomenon of 2 and over
2 kinds of virus mixed infection on cucumber was very common in Shunyi and Changping Districts of Beijing，
Shouguang of Shandong and Shenzhen of Guangdong．