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| Isolation of Heat-stable Candidate Gene Mi-9 for Resistance to Root-knot Nematode in Wild Tomato LA2157 and Construction of Over-expressive Vector |
| WANG Yin-lei 1，2，CHEN Wei-nan3，ZHAO Li-ping 1 ，2，ZHOU Rong 1，2，SONG Liu-xia 1，2，YU Wen-gui 1，2，ZHAO Tong-min 1，2* |
1 Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，Jiangsu，China；2 Key
Laboratory for High Efficient Horticulture Crops Genetic Improvement of Jiangsu Province，Nanjing 210014，Jiangsu，
China；3 School of Horticulture and Plant Protection，Yangzhou University，Yangzhou 225009，Jiangsu，China |
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Abstract Root-knot nematode disease is one of the important tomato soil-borne diseases．Breeding varieties
with root-knot nematode resistance is an effective control method to deal with this disease．But at present，Mi-1
the only gene transferred to tomato culture with resistance to root-knot nematode loses its resistance when the soil
temperature is over 28 ℃．In this study，wild tomato accession LA2157 with heat-stable resistance to root-knot
nematode was used．According to the sequence information of Mi-1，we carried out homologous cloning on Mi-9
gene with heat-stable resistance to root-knot nematode and gained 2 candidate gene segments from LA2157．The
candidate gene was ligated with the overexpression vector pBI121 by In-Fusion cloning technique．Electrophoresis
and sequencing analysis proved that the recombinant vectors of the candidate gene were constructed．
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Received: 25 August 2017
Published: 30 September 2017
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