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| Molecular Identification of Tomato chlorosis virus on Eggplant in Shandong Province |
| LIU Yong-guang 1，DAI Hui-jie 1，YANG Yuan-yuan 2，CHEN Peng 2，ZHU Xiao-ping 2，ZHAO Jing 1* |
| 1 Weifang College of Science & Technology，Key Laboratory of Protected Horticulture for Universities in Shandong Province，Shouguang 262700，Shandong，China；2 College of Plant Protection，Shandong Agricultural University，Key Laboratory for Biology of Vegetable Diseases and Insect Pests in Shandong Province，Tai’an 271018，Shandong，China |
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Abstract In order to clarify the occurrence of Tomato chlorosis virus（ToCV）on eggplant in Shandong
Province，this paper detected and identified by RT-PCR the samples of eggplant leaves prospectively infected
by ToCV and also the viruliferous percentage of ToCV in Bemisia tabaci adults on infected eggplant plants．The
results showed that 463 bp specific fragments were amplified from the detected samples and 6 samples were positive
for ToCV and the positive percentage was 60%．The CP gene of positive samples were cloned and sequenced，
sharing over 99.0% nucleotide identity with a tomato isolate from Beijing（BJ）．Moreover，ToCV could be
detected in B. tabaci on ToCV-infected eggplant plants and the viruliferous percentage of ToCV was 70%．
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Received: 28 May 2017
Published: 01 August 2017
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