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| Analysis of Glucosinolates Composition and Content in Filial Lines Derived from Inter-Varietal Cross of Savoy and Tai-tsai by HPLC |
| FENG Xi-huan1，SUN Yan1，LIU Qian-qian1，WANG Qiao-mei2，LIU Wei-xin1* |
1 College of Horticulture，Qingdao Agricultural University，Qingdao Key Laboratory of Genetic Improvement and Breeding
in Horticultural Plants，Qingdao 266109，Shandong，China；2 Department of Horticulture，Zhejiang University，
Key Laboratory of Horticultural Plant Growth，Development and Quality Improvement，Ministry of Agriculture，Hangzhou
310029，Zhejiang，China |
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Abstract To understand the variation of glucosinolates composition in backcrossing lines derived
from inter-varietal crosses in pakchoi，the content and composition of glucosinolates were analyzed by high
performance liquid chromatography（HPLC）for savoy〔Brassica campestris L. ssp. chinensis（L.）Makino
var. rosularis Tsen et Lee〕cv.‘Shanghaixiaobaye’，tai-tsai〔Brassica campestris L. ssp. chinensis（L.）
Makino var. tai-tsai Hort〕cv.‘Pingdutaicai’，and 2 lines selected from F2 populations of 4 times backcrossing of
（‘Shanghaixiaobaye’בPingdutaicai’）with‘Shanghaixiaobaye’as the recurrent parent．The results
showed that each of 4 kinds of aliphatic and indole glucosinolate were detected in the leaf and petiole of 4 tested
materials．The total contents of aliphatic glucosinolate，indole glucosinolate and total glucosinolate in leaves were
higher than petioles，and also different with 4 tested materials．The contents of health-beneficial compounds
2-phenylethyl glucosinolate（NAS）and glucobrassicin（GBC）were higher in leaves of‘6-1’than both its
parents‘Shanghaixiaobaye’and‘Pingdutaicai’，indicating that inter-varietal crosses could be used in breeding
new pakchoi cultivars with improved glucosinolate composition．
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Received: 19 September 2016
Published: 11 November 2016
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