Abstract：In order to investigate the genetic structure of srawberry mould from Botrytis cinerea，its differentiation and veriation regulatory，this paper carried out SSR marker analysis on DNA of 180 srawberry mould from Botrytis cinerea genomes，collected from 9 locations of Hunan Province．The result indicated that a total of 56 alleles（Na）for 9 SSR loci were detected，the average value was 6.22．The effective number of alleles（Ne）ranged from 1.27-4.89，with an average value of 3.44．The Nei’s gene diversity（H）varied from 0.21-0.80，with an average value of 0.65．The Shannon’s information index（I）varied from 0.37-1.83，with an average value of 1.34．The polymorphism information content（PIC）for 9 loci ranged from 0.13-0.19，the variation law was generally coincident with the Shannon’s information（I）index and gene diversity index（H）．The denote gene diversity in the species（Ht）of different SSR loci ranged from 0.21-0.77，with an average value of 0.65．The gene diversity within populations（Hs）varied from 0.19-0.50，with an average value of 0.40．The population genetic differentiation（Gst）varied from 0.10-0.46，with an average value of 0.37．The gene flow （Nm）ranged from 0.58-4.47，with an average value of 1.16．At geographical population level，the observed number of alleles（Na）and the effective number of alleles（Ne）were 2.93 and 2.07，respectively．The average Shannon’s information index（I）and Nei’s gene diversity index（H）were 0.67 and 0.40，respectively．The number of polymorphic loci（NP）and the proportion of polymorphic loci（P）were 6.78 and 75%，respectively．Based on Nei’s genetic distance（ranging from 0.279 7-1.922 5），9 geographical populations of MLP isolates from different regions in Hunan Province were initially grouped into 4 clusters：cluster Ⅰ included isolates Changsha，Shaoyang，Yueyang，Hengyang，Zhangjiajie and Xiangtan，cluster Ⅱ included isolates Chenzhou，cluster Ⅲ included isolates Zhuzhou，and cluster Ⅳ comprised Changde．The genetic diversity of Botrytis cinerea population from Hunan Province is very rich，but there are small differences between different geographical population．The genetic variation in bacteria mainly comes from inside population，and Botrytis cinerea moves between different areas．