Tuber mustard clubroot is a soil-borne disease and closely related with soil fungal community．In this study，the change regulation of soil fungal community during different occurance times of tuber mustard clubroot（pre-transplanting，premorbid after transplant，early and middle stages of disease occurance，after harvest）was sequenced by 454 high-throughout method using 18S rDNA sequence of soil fungi as target．The results showed that average of 1 056 OTUs（Operational Taxonomic Units）were obtained from one soil sample．According to taxonomy of each OTU，the dominant group was Ascomycetes，followed by Basidiomycota．The diversity indexes of Shannon and Simpson，numbers of OTU and genera in premorbid after transplanting，early and middle stages were all higher than that in pre-transplanting stage and after harvesting stage．The results indicated that the diversity of soil fungal community is higher in the period of tuber mustard clubroot development．It might be related to the vigorous production of root exudate of tuber mustard．