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| Genetic Background Screen of Inter-specific Hybrids between SSR Marker Assisted Chinese Kale and Rapeseed |
| YU Hai-long，FANG Zhi-yuan，YANG Li-mei，LIU Yu-mei，ZHUANG Mu，LI Zhan-sheng，LYU Hong-hao，ZHANG Yang-yong* |
| （Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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Abstract To screen the genetic background and increase the back-cross breeding efficiency of the interspecific hybrids and their back-cross offsprings between Chinese kale（Brassica oleracea var. alboglabra，CC，2n=2x=18） and rapeseed（Brassica napus，AACC），the F1 inter-specific hybrids and their BC1 backcross offsprings between Ogu-CMS Chinese kale and rapaseed were obtained through hand pollination combined with embryo rescue technology. A total of 220 SSR primers were used to detect polymorphisms between the parents，in which 51 pairs of SSR primers showed polymorphism. 33 pairs of primers were selected to analyze the genetic background of 3 F1 plants and 35 BC1 plants，with even distribution on 9 chromosomes and stable amplifications．The results of analysis by software NTSYSpc2.11a showed that the similarity coefficients between F1 plants and rapeseed was 0.74，which indicated that the genetic background of F1 plants was closer to rapeseed than to Chinese kale，while the similarity coefficients between BC1 plants and Chinese kale varied from 0.26-0.65，with big genetic background differences among BC1 plants．In all 35 BC1 plants，the genetic background of individual 14Y1 was the closest to Chinese kale，which was further confirmed by morphological observation．Thus，the individual 14Y1 could be used as donor plant for further backcross.
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Received: 15 March 2015
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