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| Relationship between Size of Root Knot and Quantity of Different Instars of Meloidogyne Incognita in Cucumber |
| WANG Yu-ling1，LIU Qi-zhi1*，ZHOU Hai-ying1，LI Na2，WANG Li2，TIAN Ya-ting1 |
| 1Key Laboratory for Biological Control，Ministry of Agriculture，Laboratory of Entomology and Nematology，College of Agriculture and Biotechnology，China Agricultural University，Beijing 100193，China|2College of Biological Sciences，China Agricultural University，Beijing 100193，China |
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Abstract Taking cucumber（Cucumis sativus L.）‘Qianqiu No. 1’as material and by room experimental method，according the size of root knots caused by root knot nematode（Meloidogyne incognita），this study caculated the stage and nematode numbers，and found out the relationship between them．The results showed that in solar greenhouse，there were 2-3 adults within the root knots of 2-3 mm diameter size in cucumber root system and almost each knot in this size had one egg mass．Within each larger size（over 4 mm in diameter）root knot，there were 1-5 2nd instar individuals and 2-8 egg masses．Within the knots of 16-20 mm long diameter size，42 individuals knot of adults on average could be found．After the 2nd instar root knot nematodes were inoculated into‘Qianqiu No. 1’cucumber for 45 days（inoculation rate：120 per plant），there were larger size eggs（over 5 mm in diameter），which could averagely hatch 807 individuals of 2nd instar．Larger amount individuals appeared on the 3rd and 7th hatching days．In cucumber growing fields，the quantity and occurring period of root-knot nematode can be predicted according to diameter sizes and stages，and the root knot nematode controlling time should be no later than the root knot in 5 mm diameter．In laboratories，the higher amount of the 2nd instar nematode could be obtained on the 3rd and 7th hatching days 45 days after inoculation in cucumber growing soil．
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Received: 11 June 2012
Published: 17 August 2012
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