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Isolation and Identification of Garlic Dry Rot Disease Pathogen |
LI Xu-shuang,CHEN Dian,LIANG Yu,WANG Yong* |
College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China |
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Abstract In order to study the pathogens of garlic dry rot disease,this study used the susceptible garlic(Allium sativum L.)strain Z-1001 for pathogen isolation,single-spore purification and pathogenicity determination.The results revealed that the F strain and J strain were main garlic dry rot pathogenic strains.The results of pathogen morphology observation and rDNA ITS sequence analysis showed that F strain was Fusarium oxysporum Schlecht,while J strain was Fusarium proliferatum(Matsush.)Nirenberg,which in turn revealed that F.?oxysporum Schlecht and F.?proliferatum(Matsush.)Nirenberg were the main pathogens of garlic dry rot disease.
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Received: 01 March 2012
Published: 27 March 2012
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