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Effect of Vernalization on Nucleic Acid Content of Brassica oleracea L. var. italica Plenck |
JIANG Xin-mei, WU Mei-xuan, YU Xi-hong*, LIU Sheng-cai, XU Wei |
College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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Abstract The change of nucleic acid content in broccoli Brassica oleracea L. var. italica Plenck during vernalization under temperatures in daytime/at night=(17.3±1)℃/(9.3±1)℃ was studied using cultivar‘Qingfengqinghuacai103’with 5 true leaves as experiment material. The results showed that after vernalization, the contents of DNA、RNA and nucleic acid, the ratio of RNA/DNA were higher than the control(CK), which had not be vernalized, and these contents increased with the process of vernalization. Among them, the contents of RNA and total nucleic acid, and the ratio of RNA/DNA were rapidly increased before the vernalization, while the DNA content was swiftly increased at the end of vernalization.
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Received: 22 December 2010
Published: 28 March 2011
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