Abstract To analysis submergence tolerance of 40 cucumber（Cucumis sativus L.）cultivars, the seedlings of each line at five-leaf stage were treated with flooding water. The plant death rate, chlorophyll loss ratio, SOD, POD and CAT values were checked at the 0 and 6 days after treatment. Based on the average of subordinate function（SF）analysis, North China cucumber showed high tolerance to submergence treatment and South China cucumber was middle tolerant to submergence, but zaoer N line has high tolerance to submergence. American field cucumber and European greenhouse cucumber showed low tolerance or susceptible to submergence. Two Japanese cucumber lines and one West Asia cucumber line were used in present evaluation. They showed middle tolerance and susceptible to submergence treatment.