|
|
|
| Cloning and Sequence Analysis of LFYZQ from Brassica oleracea L. var. capitata L. |
| TANG Qing-Lin, WANG Zhi-Min, REN Xue-song, SONG Ming*, WANG Xiao-Jia |
| College of Horticulture and Landscape Architecture, Southwest University, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China |
|
|
|
|
Abstract DNA and RNA were extracted from bolting stem apex of cabbage（Brassica oleracea L. var. capitata L.）cultivar ZQ and LFYZQ gene fragments were cloned by RT-PCR respectively with 2 pairs of primers. LFYZQ of DNA and cDNA sequence fragments assembly were respectively 2 560 bp and 1 239 bp. Analysis of the nucleotide sequence by BLAST on line indicated that homology of LFYZQ was up to 91 % between Brassica oleracea L. var. capitata L. and Brassica oleracea L. var. botrytis L.，up to 87 % between it and Arabidopsis thaliana, up to 86 % between it and Brassica juncea Coss., and up to 87 % between it and Raphanus sativus L. Analysis of gene structure by DNAstar indicated that LFYZQ including 3 extrons（452, 394, 393 bp）which totally coded 412 amino acids and 2 introns（514, 807 bp）which were unanimous with nucleotide splicing rules of GT-AG. Analysis of amino acids among LFYZQ and other 12 kinds of Brassicaceae LFY on line were divided into 2 classes: class Ⅰ with 3 plants（Brassica oleracea var. botrytis L., Brassica oleracea var. capitata L., Jonopsidium acaule）and class Ⅱ with other 10 plants. Molecular relativity quality of LFYZQ was 46 kD. LFYZQ was an unstable and hydrophobic protein with 4 kinds of active sites: N-myristoylation site, protein kinase C phosphorylation site, Casein kinase Ⅱ phosphorylation site and Amidation site.
|
|
Received: 12 August 2010
|
|
|
|
|
|
|
