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| SRAP Marker Analysis of Recessive Genic Male Sterile Restoring Gene in Chinese Cabbage |
| ZHANG Hui, ZHANG Shu-jiang, LI Fei, ZHANG Shi-fan, SUN Ri-fei* |
| Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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Abstract Genic male sterility lines were widely used in F1 seed production in Chinese cabbage（Brassica rapa L. ssp. pekinensis）. A recessive genic male sterile line 454AB which contained 320 individuals was constructed for this study. SRAP techniques and bulked segregant analysis（BSA）were used to screen markers linked to the RGMS restoring gene. Among the 1 128 primer combinations, only BMe10SA4 and M52K2 showed polymorphism between bulks of male sterile and fertile. The genic distances between restoring gene with BMe10SA4 and M52K2 were 4.35 cM and 7.74 cM, respectively.
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Received: 27 September 2010
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