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| Cloning and Expression Analysis of Expansion Cs-EXPA2 Gene in Dwarf Cucumber |
| WANG Duo-jia, HUANG Sha-sha, LI Feng-lan, LI Jin-yang, HU Bao-zhong |
| Life Science College of Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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Abstract In this study, we cloned an expansion gene Cs-EXPA2 from dwarf cucumber（Cucumis sativus L.）D0462 by RT-PCR. We quantitatively detected its expression in hypocotyls, roots and cotyledons within 88 h during cucumber seedling stage by Real-time PCR. The result shows that the level of its expression may be related to the state of cell growth and division in these sites, and the expression level shows obvious differences at different growth stages. The express quantity of Cs-EXPA2 changes along with temporal and spatial variation. The expression pattern of Cs-EXPA2 in hypocotyls is‘low-high-low’, and the expression pattern in roots is‘high-low-high’.
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Received: 27 July 2010
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