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Construction of ICE1 Gene Plant Expression Vector and Transformation into Tomato(Lycopercicon esculentum Mill.) |
ZHANG YU,JIANG Xin-Mei*,YU Xi-Hong* |
Vegetable Physiology and Installation Horticulture Laboratory,College of Horticulture,Northeast Agricultural University,Harbin 150030,China |
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Abstract This experiment was based on the plant expression vectorPCAMBIA3301,constructed the plant expressing vectors P3301-ICE1,Carrying an Arabidopsis thaliana ICE1 gene under the drive of CaMV35S promoter.the plasmid was transferred into tomato(Lycopersicon esculentum Mill)“cultivar Da Huang”via agrobacterium-mediated transformation.5 cultivar Da Huang with Kan-resistance were obtained,The results of test PCR amplifications of ICE1 gene and RT-PCR proved that obtain 3 positive plant of cultivar‘Da Huang’,positive plant rate was 60%.The transgenic tomato were treated with low temperature(4℃)stress for 72h,the content of MDA were apparently lowered,the content of Pro and the activityof CAT and POD were significant higher.compared with non-transgenic tomato.
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Received: 07 March 2010
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