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| Cloning of a cDNA Fragment of Acid Invertase Gene from Tomato Fruit |
| Yu Xiyan 1; Zhao Shuangyi 2; He Qiwei 3; et al. |
| 1College of Horticulture; Agriculture University; Tai′an 270118; 2College of Life Sciences; Shandong University; 3Vegetable Institute; Shandong Academy of Agricultural Sciences |
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Abstract PCR primers were designed based on the consensus domain of some acid invertase genes in GenBank. A cDNA fragment was amplified from the total RNA isolated from toamto fruit via RT PCR and cloned into pMD18 T vector. The determined sequence showed that the amino acid sequence is 99% homologous to that of the corresponding region of acid invertase gene (Access No. M81081) in GenBank. This confirmed that we cloned a cDNA fragment from tomato fruit successfully and its access number is confirmed as AF490530 i...
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Received: 28 March 2002
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