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Tissue Culture and In Vitro Propagation of Eutrema wasabia |
Wang Li 1; Long Chunlin 2 |
1College of Horticulture; Yunnan Agricultural University; Kunming 650201; 2Kunming Institute of Botany; Chinese Academy of Sciences |
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Abstract The leafstalks with buds from Eutrema wasabia were cultured in MS medium supplemented with different combinations,and under different temperatures and pH values. The results show: 1. The optimal culture media are MS+6 BA 2.0mg·L -1 +NAA 0.03mg·L -1 for induction and differentiation of adventitious buds,and 1/2 MS+NAA 0.1mg·L -1 +IBA 0.05mg·L -1 +AC 0.3% for successful propagation and differentiation of roots;2. The suitable temperature is 16℃ and the optimum pH value is 6 for Eut...
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Received: 13 July 2001
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