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| Identification of Pathogenic Bacteria from Gray Mold of Allium ascalonicum in Liaoning Province and Studies on Their Biological Characteristics |
| CHEN Le，MIAO Zeyan，SUN Baixin，ZHAO Yang* |
| Institute of Plant Protection，Liaoning Academy of Agricultural Sciences，Shenyang 110161，Liaoning，China |
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Abstract In order to clarify the pathogen of gray mold of Allium ascalonicum in Liaoning Province，this experiment isolated the pathogenic from gray mold of Allium ascalonicum collected from Xinglongbao Town，Xinmin City；identified them by molecular biology method；and studied their biological characteristics．The results showed that the pathogen of gray mold Allium ascalonicum was Botrytis byssoidea．The suitable carbon sources were glucose and fructose，and proper nitrogen sources were ammonium nitrate，yeast extract and peptone．These mycelium could grow under the condition of 10-30 ℃．The optimum growth temperature was 25 ℃．The mycelium grew slowly when temperature was over 30 ℃，and would stop growing at 35 ℃．The mycelia could all grow under the condition of pH 5.0-11.0，but the optimum pH was 7.0．Mycelial growth were sensitive to light．Under the conditions of 24 h continuous lighting and 12 h/12 h light/dark alternate lighting，mycelial would grow faster，while under condition of 24 h continuous darkness mycelial growth was the slowest．
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Received: 21 April 2022
Published: 15 July 2022
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