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Isolation and Identification of Three Strains of Pathogen Causing Konjac Stem Rot and Studies on Pathogenicity |
ZHAO Xingli1,HE Shengling1,LIU Sirui1,LUO Linli1,ZHOU Luona1,FAN Shijie1,ZHOU Yufeng 2* |
1Institute of Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China;2 Tea Research
Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China |
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Abstract Konjac stem rot caused by Fusarium spp. is one of the important soil-borne diseases during
konjac cultivation.In order to clarify the pathogens causing konjac stem rot,this experiment collected several
konjac sickened plant samples from Xingyi City,Guizhou Province in 2019;carried out isolation on these
samples by tissue separation method;identified the isolates by using morphological identification method
combined with molecular method based on rDNA-ITS and tef sequences;then proceeded pathogenicity test
according to Koch’s Postulates.The results showed that 3 strains of Fusarium pure culture were obtained
through tissue separation method.They were belonged to 2 different species,i.e.,F. oxysporum and F. solani
by identification.Further pathogenicity analysis showed that F. oxysporum xymy-8 had no pathogericity,
F. solani xymy-7 and xymy-9 were both pathogenic,and their pathogenicity was different.Among them,
xymy-7 had stronger pathogenicity than xymy-9.The experimental results had provided theoretical basis for
precise prevention and control of konjac stem rot.
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Received: 28 September 2021
Published: 13 January 2022
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