Effect of Adding Microbial Agent after Dazoment Fumigation Treatment on Fungal Diversity in Strawberry Continuous Cropping Soil
LI Yan1，QI Lianfen1，HOU Dashan2，WANG Zifan1，WANG Dandan1，LIU Lihong2，XIA Bing2，SHI Jianhua1*
1Shijiazhuang Academy of Agricultural and Forestry Sciences，Shijiazhuang 050021，Hebei，China；2Shijiazhuang Agricultural Technology Extension Center，Shijiazhuang 050051，Hebei，China
Abstract：Abstract：In order to investigate the effect of adding different microbial agents on fungal diversity instrawberry continuous cropping soil after soil disinfection，this experiment set up 7 treatments after dazometfumigation，including T1（Trichoderma harzianum + Bacillus subtilis + Actinomycetes），T2（Trichodermaharzianum + Bacillus subtilis），T3（Trichoderma harzianum + Actinomycetes），T4（CK），T5（Bacillussubtilis），T6（Actinomycetes），and T7（Trichoderma harzianum）；and analyzed the soil fungal communityand its composition by high-throughput sequencing platform（Ⅰllumina HiSeq 2500）．The results indicated that applying microbial agents could increase fungal abundance，diversity and population quantity．Comparing withT4，single application of microbial agents in T5，T6 and T7 all could increase the species abundance of fungalcommunities．While，applying mixed microbial agents in T1 and T3 could increase species abundance，but T2could decrease species abundance．The application of different microbial agents after dazomet fumigation couldincrease soil fungal diversity．Comparing with the CK，soil fungal populations of other microbial agents wereincreased by 5.62%-57.54%，except T2，where the fungal population was decreased. In turns from highestto lowest were T6，T7，T3，T1，T5，and T2．After dazomet fumigation，the treatments of microbialagents containing Bacillus subtilis，Actinomycetes，and Trichoderma harzianum could significantly increasesoil fungal populations and speed up their recovery rate，especially the populations of beneficial bacteria likeLecanicillium，Cladorrhinum，Remersonia，and Aspergillus．In summary，after dazomet fumigation anddisinfection，adding microbial agents to strawberry continuous cropping soil is a scientifically effective methodfor rebuilding microbial community balance of cultivated soil and alleviating continuous cropping obstacles．
