Identification of Pathogen of Leaf Black Stalk of Green Radish in Tianjin and Screening of Control Chemicals
BEN Haiyan1，HAO Yongjuan1*，HUO Jianfei1，YAO Yurong1，GAO Wei1，YU Jinping1，WANG Li3， WANG Wanli1，CHAI Ali2，LI Lei2，LI Baoju2*
（1Institute of Plant Protection，Tianjin Academy of Agricultural Sciences，Tianjin 300381，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，State Key Laboratory of Vegetable Biobreeding，Beijing 100081，China；3Plant Protection and Inspection Department，Tianjin Agricultural Development Service Center，Tianjin 300061，China）
Abstract：Abstract：In recent years，the‘ black stalk disease’，a new symptom of radish，had become increasinglyserious in Tianjin．The purpose of this study was to accurately identify the pathogen，and carry out fungicidetoxicity test in lab for screening of high efficiency，low toxicity fungicide in the light of target pathogens．Inthis study，the occurrence of radish ‘black stalk’ in the field in Tianjin was investigated．The pathogen was identified by morphological，pathogenicity and molecular biology．Nineteen kinds of fungicides commonly usedfor bacterial control were selected for screening by the inhibition comparison and toxicity testing．The resultsshowed that the pathogenic bacteria causing the‘ black stem disease’ of local Weiqing radish were Xanthomonascampestris pv. campestris（Pam.）Dawson，which was a new symptom of radish black rot disease．Theisolated pathogenic strains exhibited pathogenicity differentiation，among which the highly pathogenic strainLB-B was selected．The results of laboratory toxicity test showed that there were 10 kinds of fungicides withdifferent effects of inhibition on radish black rot．Among them，0.3% tetramycin AS and 10% propylthiazole SChad the best inhibition effect on radish black rot，and their inhibition rates were above 89.52% at 1 000 mg · L-1concentration．The EC50 of 0.3% tetramycin AS and 10% propylthiazole SC were 156.69 mg · L-1 and 174.04mg · L-1，respectively，which showed the stable and the long duration of efficacy in the field．The aboveexperimental results provided candidate fungicide for effective control of radish black rot disease．
贲海燕，郝永娟*，霍建飞，姚玉荣，高苇，于金萍1王丽，王万立，柴阿丽，李磊，李宝聚*. 卫青萝卜黑腐病病原鉴定及防治药剂的筛选[J]. 中国蔬菜, 2024, 1(2): 58-67.
BEN Haiyan1，HAO Yongjuan1*，HUO Jianfei1，YAO Yurong1，GAO Wei1，YU Jinping1，WANG Li3， WANG Wanli1，CHAI Ali2，LI Lei2，LI Baoju2*. Identification of Pathogen of Leaf Black Stalk of Green Radish in Tianjin and Screening of Control Chemicals. China Vegetables, 2024, 1(2): 58-67.
