Studies and Prospects for Somatic Hybridization in Vegetable Crops
YANG Dongxu，LIU Yumei，HAN Fengqing，ZHANG Shuo，YANG Lin，LI Zhansheng*
Key Laboratory of Horticultural Crop Biology and Germplasm Creation，Ministry of Agriculture and Rural Affairs，State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
Abstract：Abstract：Somatic hybridization is a technique，which can induce fusion of plant protoplasts fromdifferent species，genera or even families and then obtain regenerated plants by culture in vitro．As aneffective technology，protoplast fusion can overcome the physiological barriers to hybridization in traditionalbreeding，realize the recombination of nucleoplasmic genes between different materials，and transfer excellentgenes，create and obtain new germplasm containing target characters．At the same time，it can rapidly createmale sterile materials，and greatly shorten the breeding cycle．It is an effective means of crop breakthoughbreeding，and also a powerful tool for character improvement．The paper reviewed the development history，research and application areas of this technology on vegetables；summarized emphatically the researchprogress of protoplast separation regeneration and somatic cell hybridization in vegetable crops of Cruciferae，Solanaceae，and Apiaceae；looked forward the application prospect of somatic cell hybridization technologyin vegetable crops；and discussed the problems and countermeasures in application of this technology，so as toprovide reference for the wide application of somatic cell hybridization in vegetables．
