1Tobacco Research Institute of Chinese Academy of Agricultural Sciences，Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management，Qingdao 266101，Shandong，China；2Luoyang Company of Henan Tobacco Company，Luoyang 471000，Henan，China
Abstract：Pepper mild mottle virus（PMMoV）is a significant viral disease that occurs in peppers．In this study，we utilized reverse transcription loop-mediated isothermal amplification technology（RTLAMP）to develop a rapid PMMoV detection method．Specifically，we targeted the PMMoV coat protein CP sequence and designed four primers（F3/B3 and FIP/BIP）．The results demonstrate this method enables specific detection of PMMoV within 60 minutes，offering a sensitivity 10 times higher than traditional RT-PCR technology．Furthermore，it does not exhibit any cross-reaction with other related viruses during the detection process．The established PMMoV RT-LAMP detection method is characterized by its simplicity，strong specificity，and high sensitivity，making it suitable for rapid detection of PMMoV in field production．
