Antifungal Activity of Celery Seed Three Times Ethanol Extracts on Fusarium oxysporum f.sp. cucumerinum and Preliminary Analysis of Chemical Substances by GC-MS
（1College Agriculture，Chifeng University，Chifeng 024000，Inner Mongolia，China；2College of Chemistry and Life Science，Chifeng University，Chifeng 024000，Inner Mongolia，China；3College of Horticulture and Plant Protection，Inner Mongolia Agriculture University，Hohhot 010019，Inner Mongolia，China）
Abstract：In order to explicit the phytochemical constituents inhibiting Fusarium oxysporum f. sp.cucumerinum and contained in celery seed，this study took celery seed‘Jinnanshiqin No.1’as test material；adopted ethanol extraction method to obtain crude extract；then conducted column chromatography separation and purifi cation for 3 consecutive times；carried out test in dishes for the inhibition of Fusarium oxysporum f.sp.cucumerinum on obtained fl uid fraction；analyzed the optimal fl ow fraction obtained by the third chromatography using gas chromatography-mass spectrometry（GC-MS）；and studied the kinds and components of phytochemicals inhibiting Fusarium oxysporum f.sp. cucumerinum in the tertiary alcohols of celery seed．Results indicated that the different flow fractions of ethanol extract from celery seed obtained by 3 chromatographic separation could inhibit the mycelium growth and spore formation of Fusarium oxysporum f.sp. cucumerinum．The 4 optimal fractions were SE655，SE667，SE767 and SE777．Their inhibition rates for spore formation were 88.30%，87.82%，84.74%，86.24%，respectively；for mycelium growth were 25.21%，36.88%，35.95% and 25.81%，respectively．The GC-MS analysis showed that the kinds of bacteriostatic phytochemicals contained by the 4 optimal fractions were abundant with different contents．Tentatively，10 classes of 36 compounds were isolated and identified，including 9 kinds of alkanes，8 kinds of esters，6 kinds of organic acids，4 kinds of organic amines，3 kinds of phenolic compounds，2 kinds of aldehydes，1 kind of alcohol，1 kind of aromatic hydrocarbon，1 kind of heterocyclic compound and 1 kind of phytotoxic．Among them，the relative amounts of esters，organic amines and phenolic compounds were higher．
钱程1，赵雪梅1，郭成2，周玉雷1，秦立金1，云兴福3*. 芹菜种子 3 次醇层物对黄瓜枯萎病菌的抑菌活性及化学物质 GC-MS 初步分析[J]. 中国蔬菜, 2023, 1(6): 77-84.
QIAN Cheng1，ZHAO Xuemei1，GUO Cheng2，ZHOU Yulei1，QIN Lijin1，YUN Xingfu3*. Antifungal Activity of Celery Seed Three Times Ethanol Extracts on Fusarium oxysporum f.sp. cucumerinum and Preliminary Analysis of Chemical Substances by GC-MS. China Vegetables, 2023, 1(6): 77-84.
