Isolation and Identification of Cucumber Fusarium Wilt Antagonistic Bacteria Burkholderia gladioli L1-3 and Effect of Disease Prevention and Health Promotion
LI Yali，HOU Dong，YUE Hongzhong，ZHANG Dongqin，DUAN Yanqiao
Vegetable Institute，Gansu Academy of Agricultural Sciences，Lanzhou 730070，Gansu，China
Abstract：In order to screen out biocontrol bacteria to prevent cucumber Fusarium wilt，this experiment collected soil samples from 22 Fusarium wilt infected plots planted with cucumber in Gansu Province for successive years；screened out antagonistic strains by plate confrontation method；and carried out identification based on morphological，physiological and biochemical characteristics and gene sequencing analysis．The inhibiting spectrum of antifungal strain and its effect of disease prevention and health promotion were determined．The results showed that 6 separated and screened out strains had stronger rivalry effect to Fusarium oxysporum f. sp. cucumerinum Owen．They were identified as Burkholderia gladioli．The control effect of applying L1-3 fungicide on cucumber Fusarium wilt was 64.71%．The root system activity of cucumber seedlings，chlorophyll content，POD and SOD activities were all significantly higher than that of the contrast．Besides，L1-3 had stronger antagonism effects on all pathogenic fungi，including Fusarium oxysporum f. sp.melonis，Fusarium oxysporum f. sp. niveum，Fusarium oxysporum f. sp. conglutinans，Pythium spinosum，Botrytis cinerea，Rhizoctonia solani，Phytophthora capsici and Alternaria solani．
李亚莉，侯栋，岳宏忠，张东琴，段艳巧. 黄瓜枯萎病拮抗菌Burkholderia gladioli L1-3 的分离鉴定及防病促生效果[J]. 中国蔬菜, 2022, 1(12): 52-58.
LI Yali，HOU Dong，YUE Hongzhong，ZHANG Dongqin，DUAN Yanqiao. Isolation and Identification of Cucumber Fusarium Wilt Antagonistic Bacteria Burkholderia gladioli L1-3 and Effect of Disease Prevention and Health Promotion. China Vegetables, 2022, 1(12): 52-58.
