Abstract：Tomato yellow leaf curl virus disease is one of the important diseases for tomato．On the basis of analyzing ty-5 gene coding sequence，this experiment designed functional marker of ty-5 gene；conducted genotype identification on tomato resistant inbred line ‘t33-14’ to tomato yellow leaf curl virus（TYLCV），susceptible inbred line t2301 to TYLCV，F2 strain of inbred line ‘t33-14’בt2301’，and 26 tomato materials from different sources；and carried out phenotype correlation analysis．The results indicated that functional marker TySNP could accurately distinguish breeding parents from homozygous genotype of tomato material resistant to TYLCV，homozygous genotype of tomato susceptible materials and susceptible heterozygous genotype in hybrid progeny populations．Different genotypes could correspond better with phenotypes，and be used for effective selection for hybrid progeny．Therefore，functional marker TySNP could be used in tomato MAS breeding，and provided a marker-assisted breeding means for directional improvement of tomato cultivars resistant to TYLCV．
