Preliminary Screening and Identification of Molecular Markers Related to Sulforaphane Content in Leaf Mustard Based on BSA-SSR
LAN Mei，ZHANG Liqin，HU Jingfeng，XU Xuezhong，YANG Hongli，HE Jiangming*
Horticultural Research Institute，Yunnan Academy of Agricultural Sciences，Yunnan Branch of National Vegetable Improvement Center，Kunming 650205，Yunnan，China
Abstract：In order to obtain molecular markers related to sulforaphane content in leaf mustard，this experiment measured the content of sulforaphane in leaf mustard of F2 generation by high performance liquid chromatography（HPLC）；and constructed F2 mixed gene pool with the bulked segregant analysis（BSA）；and conducted PCR amplification using 50 pairs of primers．Two dominant markers SF12 and SF28 were sieved and obtained．SF12 amplified about size 250 bp bands from parent‘Yuanyechuncai’，low sulforaphane content pool（L）and single plant of low sulforaphane content pool．While，SF28 amplified about size 250 bp bands from parent‘Tailesijiqing’，high sulforaphane content pool（H）and single plant of high sulforaphane content pool．SF12 and SF28 correlated with the sulforaphane content in leaf mustard were verified via some single plants of F2 generation．
兰梅，张丽琴，胡靖锋，徐学忠，杨红丽，和江明. 基于BSA-SSR技术初步筛选与叶用芥菜莱菔硫烷含量相关的分子标记[J]. 中国蔬菜, 2021, 1(5): 33-38.
LAN Mei，ZHANG Liqin，HU Jingfeng，XU Xuezhong，YANG Hongli，HE Jiangming. Preliminary Screening and Identification of Molecular Markers Related to Sulforaphane Content in Leaf Mustard Based on BSA-SSR. China Vegetables, 2021, 1(5): 33-38.
