（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Bio-technology
Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China）
Abstract：Adopting high-performance liquid chromatograph coupled with tandem mass spectrometry
（HPLC-MS/MS），this study conducted initial analysis on various forms of folic aid from different tomato
species and its derivative contents．The results showed that there were distinguished differences in total folic
acid contents in tomato fruits with different genotypes from cultispecies and wild species．The total folic acid
contents of tomato cultispecies was 1 532.79-4 530.72 μg · kg-1 and that in wild species was 2 587.85-4 035.93
μg · kg-1．The total folic acid contents of 17 Solanum pimpinellifolium accessions selected based on whole
genome sequence was 1 934.81-5 050.13 μg · kg-1．Thereinto，the folic acid contents of cultispecies M82 and
wild species LA0443 were higher，being 4 530.72 μg · kg-1 and 5 050.13 μg · kg-1，respectively．The results of
analyzing 12 kinds folic acid derivatives indicated that there were certain differences existing among folic acid
derivatives types from different germplasm resources，most of them containing at least 9 folic acid derivatives．
And the folic acid variation of these 17 S. pimpinellifolium accessions were more rich：5 accessions had 12
folic acid derivatives．While，the contents of tetrahydrofolate（THF），tetraglutamyl 5-formyltetrahydrofolate
（5-F-THFGlu4），folic acid（FA）and 10-formyl folic acid（10-F-FA） were lower or even undetectable in
some germplasm resources．In general，tomato was similar with the most crops， 5-methyl-tetrahydrofolate
（5-M-THF）was the major form of folic acid in fruit，followed by 5-formyltetrahydrofolate（5-F-THF）．
Moreover，a significant negative correlation was observed between 5-M-THF and 5-F-THF．
