Abstract：Four kinds of biocontrol bacteria NSY4，NSY19，NSY50，DSN012 with measurable
restraining effect on cucumber Fusarium wilt were screened from 15 biocontrol bacterias by slab opposing
method．Taking cucumber（ Cucumis sativus L. cv Jinyan No. 4） as test material，this paper studied the effects
of 4 biocontrol bacteria substrates on cucumber growth and Fusarium wilt control．The results showed that
biocontrol bacteria substrate NSY4 could significantly increase the plant height of cucumber seedling，fresh
weight of above ground part，fresh weight of underground part，dry weight of above ground part，and total
root length and root volume，by 42.86%，26.09%，47.06%，58.82%，57.58% and 51.44% respectively，
comparing with the CK-no adding of biocontrol bacteria．In addition，the enzyme activity of matrix in
cucumber rhizosphere was significantly higher than that of the CK．Compared with the CK，the invertase，
urease，phosphatase and catalase activity increased by 16.20%，37.60%，71.88% and 5.22%，respectively．
The numbers of bacteria and actinomycetes in the treatment of adding biocontrol bacteria matrix were
significantly increased．While，the numbers of fungi and Fusarium oxysporum were reduced．The control
effect on cucumber Fusarium wilt had reached 25.00%-62.50%．Therefore，inoculating biocontrol bacteria
matrix could promote cucumber plant growth and reduce incidence rate of cucumber Fusarium wilt．Treatment
of adding biocontrol bacteria NSY4 to substrate could significantly promote cucumber plant growth，
effectively alleviate the damage of Fusarium oxysporum，and had the best control effect on cucumber
Fusarium wilt．
