Abstract:This paper selected 75 tomato varieties widely used by market or planed to enter market;and analyzed the genetic diversity of these varieties showing rich polymorphic among tomato cultivars using 48 InDel markers.Then,24 markers were chosen to construct fingerprinting of these varieties.The result indicated that 45 out of 48 primers showed better polymorphism.The genetic distance of 75 tomato F1 hybrids were 0.16-0.91 with an average around 0.52.Seventy-five F1 hybrids were divided into 5 groups.Among them group Ⅰ,Ⅳ and Ⅴ were mainly from the national company,whereas group Ⅱ and Ⅲ were mainly from the international companies.Twenty-four InDel markers covering 12 chromosomes were used to construct fingerprinting of these varieties.They could basicly recognize these 75 tomato varieties.