In the present study，based on the self-incompatibility pistil determinant SRK genes conserved sequence，a pair degenerate primer was designed to clone the SRK gene sequence in 23 self-incompatible lines and sequenced．SRK gene sequences from 23 cabbage self-incompatible lines were performed alignments analysis to NCBI database，total 10 S-haplotypes，SRK3，SRK7，SRK8，RK16，SRK28，SRK36，SRK45，SRK46，SRK58 and SRK68 were found．Among them，5 self-incompatible lines were belonged to SRK36 S-haplotypes，accounting for 22% of all self-incompatible lines．However，only one S-haplotype self-incompatible line was detected in SRK7，SRK16 and SRK58．Moreover，pollen germination on stigma and pollen tube elongation in pistil were investigated using fluorescence microscopy after pollinated between different self-incompatible lines at flowering stage．The results indicated that pollen germinated well on the stigma and pollen tube grew well in pistil of those self-incompatible lines belonging to different S-haplotypes，whereas the pollen did not germinate
on the stigma of those self-incompatible lines belonging to same S-haplotypes．The compatibility index of 20 cross-combinations of self-incompatible lines at flowering stage was also investigated．The crosses between selfincompatible lines with same S-haplotypes were highly incompatible after pollinated at flowering stage，however，those crosses between self-incompatible lines with different S-haplotypes were highly compatible after pollinated at flowering stage．All the results showed that haplotypes identification of cabbage self-incompatible lines based on the SRK gene sequence analysis was a simple，reliable and fast method for accurate cabbage cross-combination design，and was helpful to improve the efficiency of cabbage heterosis breeding．