1 College of Life Sciences，Shanxi Agricultural University，Taigu 030801，Shanxi，China；2 Plant Protection Institute，
Hunan Academy of Agricultural Science，Changsha 410125，Hunan，China
To identify the virus pathogen inducing tomato（Solanum lycopersicum L.）mosaic and curl
disease at Jinzhong City of Shanxi Province，the technology of double-stranded RNA（dsRNA）and sequenceindependent
amplification（SIA）were used．Results of SIA and sequencing showed that there was Tobacco
mosaic virus（TMV）in affected tomato leaves．Further cloning the whole genome sequence of TMV（TMVSXFQ），
the TMV-SXFQ was gained（GenBank No. JX993906），which was 6 394 bp in length and presented a
typical Tobamovirus genome organization with 4 ORFs．Sequence comparative analysis showed that the homology
of TMV-SXFQ and Tobacco mosaic virus and other isolates was 86.3%-99.6%．Phylogenetic analysis suggested
that TMV-SXFQ isolates had closeer ties consanguinity with IM strain from South Korea and these 2 isolates formed
an independent branch．
