Analysis of Sulforaphane in Chinese kale（Brassica albograbra L. H. Bailey） by HPLC
LI Zhan-sheng1，LIU Yu-mei1*，FANG Zhi-yuan1，YANG Li-mei1，ZHUANG Mu1，ZHANG Yangyong1，LI Ling-yun2，SUN Pei-tian1
1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2 Supervision
and Testing Center for Vegetable Quality，Ministry of Agriculture，Beijing 100081，China
Abstract：Forty four lines of Chinese kale（Brassica albograbra L．H．Bailey）including 23 high inbred
lines and 21 F1 generations were used to detect the sulforaphane contents in flower stalk of Chinese kale by RPHPLC，
and to analyze the changing rule in sulforaphane contents．The results showed that the changing range
of sulforaphane contents in 44 Chinese kale was 46.89-419.45 mg·kg-1（DW），and there were significant
differences between sulforaphane contents of the testing lines．Four lines were detected with higher sulforaphane
contents，among them one line was F1 generation A86 with 419.45 mg·kg-1（DW）sulforaphane content，the
other 3 were inbred lines A1，A17 and A53．Their sulforaphane contents were 298.50 mg·kg-1（DW），354.19
mg·kg-1（DW），361.06 mg·kg-1（DW），respectively．They could be used for breeding of Chinese kale in
anti-cancer and marketing promotion．
