In 2014，severe interveinal chlorosis of typical Tomato chlorosis virus Tomato chlorosis virus （ToCV） symptom infections were observed in greenhouse tomato plants in Zhengzhou，Jiyuan，Xinzheng，Zhongmou and Fugou region of Henan Province. The disease samples from these 5 regions were collected，and a Reverse Transcript-PCR assay was used to detect the virus with total RNA isolated from the disease samples by specific primers of ToCV HSP70h. About 460 bp PCR product was obtained. The PCR products were sequenced and analyzed. The result showed that they had the highest identity（＞ 99%）with that of ToCV. This result also indicated that ToCV is one of the main chlorosis disease pathogen in tomato of Henan Province.