Studies on 4 Tomato Varieties Tolerance to NaCl Stress under Tissue Culture Condition
WANG Ping1，MENG Xiao-qin1，ZHONG Ying1，2，WANG Gang2，JI Jing2
1School of Marine Science and Technology， Huaihai Institute of Technology， Lianyungang 222005， Jiangsu，China； 2School of Environmental Science and Engineering， Tianjin University， Tianjin 300072， China
Abstract：Using hypocotyls of tomato（ Lycopersicon esculentum Mill.）varieties‘Baiguoqiangfeng’，‘Hongniuxin’，‘Xiaohongxiantao’ and ‘Hongshengnv’ as explants，we added 0 mmol·L-1，50 mmol·L-1，100 mmol·L-1，150 mmol·L-1 and 200 mmol·L-1 NaCl in the media，and investigated the changes in induction rates of callus formation and adventitious bud under the stress of different NaCl levels．We also evaluated tolerance of 4 tomato varieties to NaCl stress under the condition of tissue culture．At the same time，we probed into the effects of chlorine fumigation time on tomato seed’s growth and germination，when it was used in tomato seed disinfection. The results showed that 7 h chlorine fumigation for tomato seeds had the best effect. The capacity of hypocotyls tissue culture of 4 tomato varieties and their tolerance to NaCl were different．The induction rates of callus formation and adventitious bud of‘ Baiguoqiangfeng’ and‘ Hongshengnv’ were relatively high．100 mmol·L-1 and 50 mmol·L-1 NaCl were suitable for‘ Baiguoqiangfeng’ and‘ Hongshengnv’，respectively when NaCl was applied as selective agent to screen transformants.