Abstract：With Brassica oleracea L. var. acephalea DC. variety‘Baikongque’as experimental material，by homology cloning and RACE method，we isolated from low temperature induction of Brassica oleracea L. var. acephalea DC. seedlings the full gene sequences of Brassica oleracea L. var.acephalea DC. glycerin-3-phosphate acyltransferase（GPAT） gene，and analyzed the sequences for bioinformatics by biology software．The results show that this gene is 1 558 bp in length，speculated encoding 451 amino acids，and is named BaGPAT．The BaGPAT gene sequence has 54%-85% similarities in amino acids，when compared with other species in sequencing analysis．The results of clustering analysis show that the genetic relationship of BaGPAT，Arabidopsis thaliana GPAT and Lepidium latifolium GPAT is closer.