Detection of Cucumber Green Mottle Mosaic Virus of Watermelon Blood Flesh in Jinan Area
LIU Ying1,2,PENG Bin2,YANG Xiao-hong1*,LI Xiang-dong3,GU Qin-sheng2*
1Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China|2Biological Technology Center,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China|3College of Plant Protection,Shandong Agricultural University,Tai’an 271000,Shandong,China
Abstract:The blood flesh of watermelon〔Citrullus lanatus(Thunb.)Matsum. et Nakai〕out broke on grafted watermelon in Jinan area,Shandong Province in 2011.The viral pathogen was suspected as its cause after survey in fields.Eighty samples of grafted watermelon leaves and fruits with viral symptoms and non-symptom collected from Jinan area,Shandong Province were detected by ELISA and RT-PCR.The results showed that 70 of these 80 samples were infected by Cucumber green mottle mosaic virus,preliminary confirming that CGMMV was the cause of blood flesh of watermelon in Jinan area,Shandong Province.