Optimization of Regeneration System for Diploid Potato Anther Culture
LI Wen-xia1，NING Hai-long1，ZHANG Yong-gen2，BAI Ya-mei3，SHI Ying1，WEI Qiao-rong1，ZHANG Li-li1，LV Wen-he
1College of Agronomy，Northeast Agricultural University，Harbin 150030，Heilongjiang，China|2College of Animal Sciences，Northeast Agricultural University，Harbin 150030，Heilongjiang，China|3College of Resources and Environmente Sciences，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Abstract：In this experiment，factors and levels related to anther culture were selected to discuss the optimization of regeneration system for diploid potato（Solanum tuberosum L.）anther culture．The results indicated that the optimal genotypes for diploid anther culture were BD40-2，BD54-8 and BD10-7．Four pretreatments including low temperature，heat shock，decentralization and mannitol had significant differences on callus inducing．And the optimal combination were low temperature for 6 days, heat shock for 4 days，centrifugation for 4 000 r?min-1 and mannitol for 2 days．The horhome combination was 2,4-D 1.0 mg?L-1 + 6-BA 0.1 mg?L-1，which has the highest rate of anther induction．Conformable carbon source was barley sugar at the level of 3%．Adding 1 mmol?L-1 STS in medium had advantages in callus inducing over 50 mg?L-1 AgNO3.
