Abstract：Agrobacterium tumefaciens mediated transformation（ATMT）was applied in this research．Agrobacterium tumefaciens，which carried plasmid PCH-sGFP harboring the green fluorescent protein（gfp）gene and hygromycin phosphotransferase gene（hph），was used to transform Fusarium oxysporum f.sp. conglutinans race 1 and 2．The transformants were randomly selected to confirm．The results indicated that transformants were integrated with exogenous T-DNA in genomes successfully，and gfp gene was stable after 6 successive subcultures．The growth rates analysis and pathogenicity showed that there were no significant differences between wild isolates and transformants on growth rates and pathogenicity．So transformant can be used as a useful tool for further function research，such as histopathology and pathogenesis.
