Identification of Cabbage‘Xiyuan No.4’Seed Purity by SSR Markers
MIAO Ming-jun1, 2, LI Cheng-qiong1*, SI Jun1, REN Xue-song1, SONG Hong-yuan1
1College of Horticulture and Landscape Architecture, Southwest University, Key Lab in Olericulture of Chongqing, Key Lab of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China; 2Horticulture Institute, Sichuan Academy of Agricultural Sciences Chengdu 610066, Sichuan, China
Abstract：Cabbage（Brassica oleracea L. var. capitata L.）‘Xiyuan No.4’and its parent were analyzed by SSR molecular marker technology. A primer, with obvious different bands parent, O112-G04 was screened from 90 pairs of SSR primers. The DNA fingerprinting of cabbage‘Xiyuan No.4’was constructed, and its seed purity was identified. The result is consistent with those obtained in the field tests. The seed purity was 96.7 %. This result shows that it is reliable to use this pair of SSR primer to identify cabbage‘Xiyuan No.4’seed purity.
