Abstract：Effects of seed priming with Gentle B and Safe B on tomato（Lycopersicon esculentum Mill.）seed vigor were investigated by Q2（oxygen sensor）technology. Germination tests in common temperature（25 ℃）and temperature stresses（35 ℃/10 ℃）were conducted, so as to evaluate the feasibility of using Q2 technology to test seed vigor after priming. The results showed that both Gentle B and Safe B priming could improve seed vigor and Safe B priming showed more obvious effects. Primed seeds could shorten the increased metabolism time（IMT）and relative germination time（RGT）, increased oxygen metabolism rate（OMR）, which was accordant to germination energy and germination index. This result suggested that it was feasible to test the vigor of primed tomato seeds by Q2 technology.