Abstract：his paper mainly studied chitin degradation effect of different combination of several microbes（Bacillus spp. and Saccharomyces spp.）and control effect of chitin degradation liquid（CDL）on tomato（Lycopersicum esculentum Mill.）disease of root-knot nematodes by artificially inoculating Meloidogyne incognita. The results indicated that mixture of 4 Bacillus strains and a Saccharomyces strain had best degradation effect on chitin with the lowest viscosity, the highest chitinase activity. CDL could control tomato root-knot nematodes disease effectively, decrease root-knot rate and root-knot index. Therefore，control effect was 54.29 %, which was 2.58 times compared with the treatment of chitin liqiud medium. CDL could also improve the populations of bacteria, fungi and actinomycetes in soil.
